вторник, 21 января 2020 г.

The Fashion Revolution of 1789-1799 Essay -- essays research papers f

  Ã‚  Ã‚  Ã‚  Ã‚  Between 1789 and 1799 France was going through great turmoil. Immense political and social upheavals were commonplace in the changing nation. New ideas were growing, ideas of freedom, government and of self-expression. Such new attitudes by the people gave rise to a new style of clothing. The clothing became more expressive and more ornate. People began to dress the way they wanted. French soon became the center stage for fashion. The French revolution, while failing in many aspects did one thing no one expected. The French revolution created a new image of fashion in France. There are several reasons that contributed to this occurrence. New beliefs of freedom, equality and self-expression each contributed to this new era of clothes.   Ã‚  Ã‚  Ã‚  Ã‚  One major contributor to this new image in France was the new belief in freedom. This was a time in France were the tides were changing and the power over the country was moving from the hands of the Old regime to the Bougiose. As this occurred this working class felt a new freedom, and a respect for freedom that they had never felt before under the iron fists of the nobles. Such ideas broke the people away from their normal standards and every day drab. With a new freedom new ideas started to pop up everywhere. These new ideas led to the creation of â€Å" ‘the new costume’†¦ reproduced in England’s The Home Circle† (Ewing, 123). With the new freedom people of all sorts started to speak up and voice their desires. This caused a revolution in fashion for France with the invention of the sewing machine and other cloth production machines the ideas quickly became â€Å"vast quantities of fabrics† (Ewing, 105). As restrictions g ave way to freedom many old ideas gave way to new ones. With this newfound freedom women’s wear especially went through â€Å"a slow reform for women† (Ewing, 173). While corsets had been used earlier to give women a narrow waste and flatter chest, new half corsets were used to provide a â€Å"round, high waist† (Cassin-Scott, 74) while still providing the full chested appearance. This new freedom allowed men and women alike to change their image from a more strict and prudent society, to a more open and casual fashion. As the people gained more freedom so did the style of dress. The bustle gowns were a new style where â€Å"the curve between breasts and waists and waist... ...uality allowed self-expression to set in and mold the old styles into new ones more befitting of the people who wore them, and allowed the people to express themselves, being no longer limited by the boundaries of class. Ideas of freedom, equality, and self-expression from the French Revolution caused another revolution on its own, a revolution of image and style never before seen. The affects of the revolution even outlived the revolution itself, and were some of the few changes in France that actually remained the same, while most of the changes from it retrograded. The Revolution of fashion in the decade between 1789 and 1799 would not have occurred without the French Revolution, and it changed styles in the world forever. Bibliography:  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Cassin-Scott, Jack. French Costume and Fashion 1550-1920. New York. Brandford Press Ltd, 1986  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ewing, Elizabeth. Everyday Dress in France1650-1900. London. B.T. Badsford, 1984  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ribeiro, Aileen. Fashion in the French Revolution. London. B.T. Badsford, 1988  ·Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Yarwood, Doreen. European Costume. New York. Bonanza Books, 1975

понедельник, 13 января 2020 г.

Grade 10 english Essay

Compare and Contrast the relationship between the Students and their Teacher in â€Å"Students† and â€Å"Crow Lake. † Both the poem â€Å"Students† by Tom Wayman and the short story â€Å"Crow Lake† by Mary Lawson highlight the challenges that teachers face with their students. While Wayman explores the generation gap between himself and the kids in his class, Lawson provides an anecdote of a lecture where she suddenly realizes that she is not cut out for teaching. From the two pieces we learn how important relationships can be when given the responsibility of teaching students. Firstly, the autobiographical poem begins with the teacher being shocked at the age of the students: â€Å"Wayman was sure the computer was in error† (line 3). This reaction foreshadows the generation gap that makes it difficult for him to communicate with the teenagers in his class. Furthermore, when he says, â€Å"The wisdom of the students/ hadn’t altered, though,† (lines 9-10) we see that he respects the ability of the students; he just questions their motivations for learning. Wayman uses a series of metaphors like â€Å"The Vaccination Theory of Education,† and, â€Å"The Dipstick Theory,† to show how students learn in different ways. The tone of the lyric poem if slightly satirical but, from the ideas explored by the author, we start to appreciate the challenges that he faces coping with children from this generation. Similar to Wayman, Lawson faces challenges with her University students. While Wayman remains positive and continues to battle against this gap, Lawson has an epiphany and realizes that she has been â€Å"boring† her students. While Wayman is an experienced teacher, Lawson is more of a research expert and, as she identifies, she finds, â€Å"it hard to relate to students† (p14). Lawson uses flashback to show her passion for nature and, as she realizes that the students have not had the same experience and that is why they are indifferent to her lesson, she realizes it is her fault that they are not engaged: â€Å"And now I was putting the entire class to sleep† (p16). Unlike Wayman, Lawson decides to leave the lecture hall and, we can assume, she makes a decision that will end her teaching career. By the end of the story Lawson has realized her limitations. To conclude, both texts imply that it can be very difficult for teachers to relate to students. In a world where education is so important, we learn that the teacher has a responsibility to be creative and inspire students to learn rather than just assess them with a letter grade or drag them through material in a way that is very boring.

суббота, 4 января 2020 г.

Nazi Germany During World War I - 924 Words

After World War I, Adolf Hitler formed the Reich Ministry of Public Enlightenment and Propaganda after his rise to power in Germany. Hitler selected Dr. Joseph Goebbels as the head of the Reich Ministry to promote propaganda. â€Å"The Fà ¼hrer or Mr. Hitler explained in his book Mein Kampf why propaganda should be used to control the populace and how it had been a useful weapon for the allies during World War I. Hitler continued by criticizing the German leadership for failing to use propaganda in any meaningful way during the war, and described how the new regime could use it to its fullest power.† (Stout) Many historians believe that propaganda was Hitler’s most powerful weapon employed by Nazi Germany during World War II. What exactly was this powerful weapon? Merriam-Webster defines propaganda as â€Å"an idea or statements that are often false of exaggerated and that are spread in order to help a cause, a political leader, a government, etc.† (Merriam-Webs ter). Dr. Goebbels took charge of every means to spread a Hitler’s message. â€Å"Art was no longer just paint to canvas; it played an important role in communicating Hitler’s message. â€Å"Art became a tool for communication and expression Adolf Hitler and the Third Reich vision of a cultural cleansed race.† (Dinsmore) All content that did not support the Nazi’s vision was censored or destroyed. â€Å"Goebbels worked to inflame the anger of the Germans over their defeat in World War I and emphasized German cultural and military achievementsShow MoreRelatedThe Causes Of World War Two. On June, 28 1919, The Treaty763 Words   |  4 PagesOf World War Two On June, 28 1919, the Treaty of Versailles was signed. This treaty coupled with other factors, such as the Nazis rise to power in Germany, Europe’s policy of appeasement, and Germany’s invasion of Poland would lead to - and be direct causes of - World War II. In fact, when French military commander Marshal Foch heard of the signing of the Treaty of Versailles, her observed with extreme accuracy - â€Å"This is not Peace. It is an Armistice for 20 years.† (Churchill, 7) World War I officiallyRead MorePutzier 1. Tessa Putzier. Ms. Jeanne Bitz . Language Arts.1293 Words   |  6 PagesOf World War Two On June, 28 1919, the Treaty of Versailles was signed. This treaty coupled with other factors, such as the Nazis rise to power in Germany, Europe’s policy of appeasement, and Germany’s invasion of Poland would lead to - and be direct causes of - World War II. In fact, when French military commander Marshal Foch heard of the signing of the Treaty of Versailles, her observed with extreme accuracy - â€Å"This is not Peace. It is an Armistice for 20 years.† (Churchill, 7) World War I officiallyRead MoreWhy Was A Regime Was Or Was Not Democratic During The Time That World War I? Essay1695 Words   |  7 Pages Whether a regime was or was not democratic during the time that World War I, World War II, and the Cold War took place has no influence on being the exact cause of the war, because the determining factors of why the previously listed wars occurred lies among the many constraints; protecting alliances, attempts at deterrence, balancing power, acting on misinterpretation, rise in aggression, and difference of beliefs and ideologies in relation to those of people from other countries. It just so happensRead More A German Perspective on World War Two Essay1618 Words   |  7 PagesA German Perspective on World War Two In Germany, they first came for the communists, and I didnt speak up because I wasnt a communist. Then they came for the Jews, and I didnt speak up because I wasnt a Jew. Then they came for the trade unionists, and I didnt speak up because I wasnt a trade unionist. Then they came for the Catholics and I didnt speak up because I wasnt a Catholic. Then they came for me -- and by that time there was nobody left to speak up. -Martin Niemà ¶ller Read MoreWhy Was Germany Most Significant Influential Country During World Wars So After Losing World War II?1429 Words   |  6 PagesWhy was Germany most significant influential country during World Wars even after losing World War I and World War II? By: Kinjal Shah 12TH February 2017 University of Massachusetts Lowell Abstract: Germany’s Role in both World War had been pivotal and there are several reasons to why that happened and various facts to support the idea that it had influenced whole of the Europe. Hitler was appointed chancellor by the President of Weimar republic on 30 January 1933. The Nazi Party thenRead MoreThe Treaty Of Versailles During The First World War1440 Words   |  6 PagesThe Central Powers and Germany were defeated by the Allied Powers in the First World War. The Armistice was signed on 11 November 1918 putting an end to the war. On June 28, 1919, Germany was forced to sign the Treaty of Versailles (Thompson 548). The treaty demanded Germany to take full responsibility of all losses and damages which had occurred during the war. Also, the treaty called for the disarmament of Germany s military force, territorial concessions, and war reparation payments. AlthoughRead MoreThe Perceived Perception Of Propaganda968 Words   |  4 PagesQuotes†). During World War II, th e economy in Germany was struggling severely and the Germans wanted someone to blame. Under Hitler’s rule, he gave Germany just that, causing the persecution of millions of Jewish people. During the Holocaust, the Nazis used propaganda as a tactic to help people deceive themselves; Germans, Jews, and people from all around the world were affected by this propaganda, among them, a Jewish survivor, Martin Kapel, whose life was thrown off course and impacted by Nazi idealsRead MoreThe Nazi Regime Was Defined By Its Fascism Policies1345 Words   |  6 PagesThe Nazi regime was defined by its fascism policies in Germany that had on huge implications on the country s socio-political and economic settings. The National sozialistische Deutsche Arbeiterpartei (Nazi) evolved from the German Worker s Party. Adolf Hitler was the man behind the establishment of the Nazi party into the German political system as he rose to power in the late 1920s. He was the leader of the Nazi party. Th e party came to being from the Weimar Republic, which came about after GermanyRead MoreGermans Into Nazis by Peter Fritzsche Essay791 Words   |  4 Pages ‘German’s Into Nazis’ by Peter Fritzsche 1) Germany before the Fuhrer. Germany’s defeat at the end of World War I left the nation socially, politically, and economically shattered. The reparation agreements inflicted upon Germany without its’ consent at the end of the war meant that the nation was in complete financial ruin. In the wake of Germany’s defeat, public decent climaxed on the 9th November 1918 during the revolution that took place on Berlin’s Postdamer PlatzRead MoreThe Holocausts Effect on the German Jew Essay1745 Words   |  7 Pages Adolf Hitler came to power over Germany in January of 1933. He hated Jews and blamed them for everything bad that had ever happened to Germany. Hitler’s goal in life was to eliminate the Jewish population. With his rise to power in Germany, he would put into action his plan of elimination. This is not only why German Jews were the main target of the Holocaust, but why they were a large part of the years before, during, and after the Holocaus t. Hitler’s â€Å"final solution† almost eliminated the

пятница, 27 декабря 2019 г.

Mobile Phones Are More of a Nuisance Than a Benefit....

‘Mobile phones are more of a nuisance than a benefit.’ Discuss The mobile phone is a device which has revolutionized the world of technology. In fact the mobile phone started with the keypad buttons and has now attained the ‘touch screen’ system. Nowadays three persons out of five possess a mobile phone. It is a tool which is used everyday and is sometimes essential for someone depending on the type of job, one does. For instance, businessmen use their mobile phones much for transactions or for teleconferencing. However, it is difficult to say whether the mobile phone is more of a nuisance than a benefit. It is a controversial issue as like every technology, it has its drawbacks. Mobile phones have a wireless communication system†¦show more content†¦Chances of Alzheimer’s disease, ear defects and blurring of vision are noticed to be higher in cell phone users. In addition, cell phones damage key brain cells as well as DNA. At school mobile phones distract students. Pupils do not follow in class as they are busy playing with their phones sending text messages, or transferring data of all sorts through the Bluetooth feature. As a result of this excessive us of mobile phones, the academic performance of the students is quite diminished. In fact, a mobile phone ringing in class undoubtedly hinders students in their study. At home it is the family life which is the victim of mobile phones as children as well as parents forsake it. They spend most of their time with their cell phones. In some cases communication is thoroughly absent among members of the family. The excessive use of mobile phones directly attacks the social life of somebody. Due to such popularity, companies are constantly coming up with new software, features and models to attract more buyers and make higher profits. Hence the public would want to install new features and keep their handsets up to date, spending more money and time this way. They could also be tempted into getting hold of the latest phones even if their old ones are functioning, incurring unnecessary expenses without consideration. Furthermore, using mobile phone while driving undeniably increases the risk ofShow MoreRelatedCell Phones Harm Students Academically Essay1663 Words   |  7 Pagescomputer or tablet at their fingertips, students prefer to use a mobile phone for projects, most adults wouldn t undertake without a keyboard and mouse† (Wells D.1). Efforts to incorporate cell phone in the classroom is quickly gaining momentum (Compoy and Harte A.3). In a 2013 poll of 2600 schools, 10 percent of the schools were allowing to use mobile devices this is up from 3 percent in 20 10 (Compoy and Harte A.3). Students of today seem more connected to their wireless devices. For many years teensRead MoreRiordan Proposal Network and Data Upgrade Essay examples4613 Words   |  19 PagesRirodan Manufacturing Telephony and Data Network Upgrade Proposal Team A is proposing a thorough upgrade to Riordan’s phone and data networks. Our proposal will cover a description of the new system, its components and benefits to Riordan, as well as explain the requirements driving the need for the upgrade. We will cover the information used in the phone and data upgrades and discuss any security and ethical concerns of the system. We will be providing Riordan with diagrams of information flow andRead MoreFreemium Business Model21065 Words   |  85 PagesFreemium as a Business Model for Mobile Video SERGEJ KOTLIAR Master of Science Thesis Stockholm, Sweden 2011 Freemium as a Business Model for Mobile Video SERGEJ KOTLIAR Master’s Thesis in Media Technology (30 ECTS credits) at the Media Management Master Programme Royal Institute of Technology year 2011 Supervisor was Christopher Rosenqvist, SSE Examiner was Johan Stenberg TRITA-CSC-E 2011:026 ISRN-KTH/CSC/E--11/026-SE ISSN-1653-5715 Royal Institute of Technology School ofRead MoreSocial Networking Sites-Boon/Bane15517 Words   |  63 Pagesit has got a different dimension. I dont see anything wrong with it. Every generation has its own way of communicating. We cannot judge good or bad behaviour through it, but its the actual behavior which should be a source of concern for them than any secret language, It can also be a source of a rebel cause for a generation that wears Che Guevera T-shirts as Facebook addict Avirat Akre says, Its the Gen-X way of breaking rules when they mistake parenting for pestering and tend to rebelRead More The Ethics in Securing the Internet Essay4456 Words   |  18 PagesInternet Introduction In order to understand the concept of internet security and why it is necessary it would be worthy of analysis to discuss what would happen if not enough of it is provided, meaning that the levels of security are not utilized to the full extents. This is exactly where the big issue of hacking and hackers becomes involved. Hackers can benefit from a wide range of weaknesses in security, break into systems, find the information they need, and eventually change them to fulfillRead MoreData Storage Using Nano-Technology and Electronics5235 Words   |  21 Pagesstorage device integrated with a universal serial bus(USB) interface. Flash is based on the floating gate concept, essentially a modified transistor. USB flash drives are typically removable and rewritable, much shorter than a floppy disk (1 to 4 inches or 2.5 to 10 cm), and weigh less than 2 ounces (60 g). Storage capacities typically range from 64 MB to 64 GB with steady improvements in size and price per gigabyte. Some allow 1 million write or erase cycles and have 10-year data retention, connectedRead MoreManagement and Business22027 Words   |  89 Pagessome form of IT. Further, more employees are becoming reliant upon IT for some of their daily tasks or even may perform the majority of their daily work using IT. However, IT has not only become pervasive in the workplace: IT can also commonly be found in the home and in public areas. In fact, by the end of the 1990s, these technologies had become so commonplace that airplane passengers today are instructed as to when the use of computers and cellular phones are and are not permittedRead MoreNebosh Diploma Note Unit C22654 Words   |  91 PagesNon-Destructive Testing Techniques –Advantages Limitations ................................................. 37   Page 3 of 89 Non-Destructive Testing – Purpose, Principles, Advantages Disadvantages............................ 38   NDT – Principles, Benefits Limitations – Dye Penetrant Radiography ................................... 39   CE Marking – Supply of Equipment ............................................................................................... 40   Thorough Examination – LiftingRead MoreThe Role of Advertising in Marketing Communications9872 Words   |  40 Pagesfrom dealers is doubtful: In many cases, the dealers do not cooperate in providing the merchandising support nor do they pass on any benefit to consumers. The retailer might not be willing to give support because he does not have the place, or the product does not sell much in his shop, or may be he thinks the effort required is more than the commission/benefit derived. 4. Short-term orientation: Sales promotions are generally for a short duration. This gives a boost to sales for a short periodRead MoreMarketing Management 14th Edition Test Bank Kotler Test Bank173911 Words   |  696 Pagesimportance when products are standardized. B) It can help create jobs in the economy by increasing demand for goods and services. C) It helps to build a loyal customer base but has no impact on a firms intangible assets. D) It is more important for bigger organizations than smaller ones. E) It is seldom used by nonprofit organizations. Answer: B Page Ref: 4 Objective: 1 Difficulty: Easy 2) ________ is the art and science of choosing target markets and getting, keeping, and growing customers

четверг, 19 декабря 2019 г.

Ban Increasing Independence With Limits - 1648 Words

Allow Increasing Independence with Limits Much research shows that adolescents do best when parents set reasonable, age-appropriate rules and expectations, and follow through with reasonable consequences for breaking rules (Simpson, 2001). Clearly stated rules and predictable consequences for breaking rules are especially important in the area of risky behavior. To the extent that it has been tested among AI youth the principle still applies. For example, Lonczak et al. (2007) found that more limit setting predicted less substance use among 13-19 year old AI / Alaskan Native youth. AI adolescents who use alcohol also report fewer parental sanctions against alcohol use than AI youth who are non-users (Oetting et al., 1988; Swim, Beauvaid, Walker, Silk-Walker, 2011). This is perhaps especially true during early adolescence (Swim et al.), a time period when initiation of alcohol use poses special risks for long-term use and dependence (Henry et al., 2011). In Mmari et al.’s (2010) qualitative study, tribal members identified lack of appropriate parental discipline as one of the most important family risk factors for youth delinquency and violence. These and other authors point out that AI parents in recent generations have not had good role models for parental discipline due to the long-term impact of children having been taken from families and raised in boarding schools. These children, once parents themselves, were not able to draw from experiences of traditionalShow MoreRelatedGun Control : A Part Of American Lifestyle Since Independence1369 Words   |  6 PagesGun culture is considered a part of American lifestyle since independence. People use and carry guns in everyday life for many purposes i.e. hunting, self-defense, sport etc. (Carter) On December 14, 2012, in Newtown, Connecticut, a 20 years old boy Adam Lanza shot and killed 20 school children and 6 staff members at Sandy Hook Elementary School, after shooting his mother at his home. At arrival of police, he shot and killed himself too (Edition.cnn.com). This incident left whole of America inRead MoreMobile Phone and Children1439 Words   |  6 Pagesin certain contexts. Mobile phones encourage the development of independence and interpersonal skills Education is as much about the growth in character and dealing with risks as it is the accumulation of knowledge; mobile phones provide for children a means to converse with peers, develop friendships and resolve disputes, all within minutes of each other, night and day. For them, ‘getting a cell phone is a step towards independence and a status symbol among their friends’. The confidence and self-esteemRead MoreGovernment Interference in Our Private Lives Essay1216 Words   |  5 Pagesgovernment have the right to interfere in people’s private lives? Democracy guarantees freedom. One might then argue that a government should allow people to act according to their own free will. But, there are always two sides to everything. Absolute independence might not lead to anything productive in a society like ours. There needs to be a basic framework of rules and guidelines in which all participants in society should agree to. According to Thomas Hobbes, â€Å"human nature is that if there were noRead MoreIncreasing Oil Production in the United States of America1306 Words   |  6 PagesIncreasing Oil Production in the United States of America Crude oil is a major source of energy for the world. Everyday the United States consumes more crude oil than it produces. The growing number of barrels of oil imported into the Unites States and rising gas prices are major concerns. Even though increasing the United States crude oil production may not lower gas prices immediately, it will eventually allow the United States to be energy independent. Oil is used in many different waysRead MoreCharacteristics Of A Free Market Economy1548 Words   |  7 Pages There are several ways in which market failure can be addressed - all involving government intervention. This includes market liberalisation, government provision of public goods, and methods in increasing information flow. However, the strategies of subsidies, taxation and bans will be the main focuses. Minimum Prices, as a type of subsidy for controlling prices can often be used as a method to address market failure resulting from factor immobility and equity issues. Sugar is anRead MoreHydraulic Fracturing And Its Effects On The United States2334 Words   |  10 PagesIntroduction Hydraulic fracturing is an increasingly common practice used to access otherwise unobtainable oil or natural gas reserves. While it shows promise as both a valuable economic resource as well as a source of energy independence, many oppose the practice due to alleged environmental dangers. In order to foster a greater understanding of the issues at hand, the following topics are discussed: what hydraulic fracturing is, how it is performed, and where it is located in the United StatesRead MoreWomen Of Saudi Women s Rights1663 Words   |  7 Pagesdeserve to be rid of many constraints and limitations enforced upon them falsely under the name of Islamic religion. Ban of women to drive in Saudi Arabia is considered a violation of rights, not only in the US but the whole world in general sees it that way, including the majority of Saudi’s. The government has banned its women from driving arguing that the reason behind the ban is that it is against islamic religion for them to do s o. This is one of the most popular controversial issues in my countryRead MoreGun Control And The Second Amendment994 Words   |  4 Pagespreserve their independence. And thus the second amendment was born which gave us the right to keep and bear arms. Another important statement to that amendment is, â€Å"Shall not be infringed upon.† This means is should never be violated or changed. Of course times are different now, but if guns are banned who is going to protect us when our freedom is being threatened once again. People often say we should not judge all of one type of person based on the actions of a few. A main argument to ban guns is massRead MoreGun Control And The American People1236 Words   |  5 Pages (LII). Having won their freedom only eight years earlier from what the Kingdom of Great Britain through an armed revolution, the founding fathers believed an armed citizenry was needed to protect the people from a tyrannical government. The independence of the United States was only won because the original colonies had established militias for their protection and most able bodied men were armed and had some military training. They knew through recent experience that they only way to guaranteeRead MoreEssay about The Revolutionary War1417 Words   |  6 PagesDevelopments After the American Revolution ended there was a debate on if all ties should be broken with Britain. Support for American Independence rode strong up into 1776. Thomas Paine wrote a pamphlet, Common Sense. This pamphlet rebelled against King George III and argues for complete Independence of the American colonies. On July 4, 1776, a Declaration of Independence was adopted stating the colonies freedom from Britain. The time after the Revolution was a period of political reconstruction.

среда, 11 декабря 2019 г.

Bacillus Subtilis-Free-Sample for Students--Myassignmenthelp.com

Question: Discuss about the Bacillus Subtilis. Answer: Introduction Bacillus subtilis is a Gram-positive non-pathogenic bacillus involved in the formation of heat resistant and dormant spores (Logan, Niall and Paul). It is the most characterized microorganisms among the various Gram-positive bacteria present. It genetic material can easily be subjected to manipulation and as a result widely used in genetic engineering. It is used commercially for the production of a variety of enzymes, vitamins, flavoring agents and in the production of industrial nucleotides (Capozzi et al.). Genomic DNA libraries carry the entire genomic DNA sequence of the organism. The genomic DNA library can be used to determine the whole genome sequence of an organism helps to determine the phenotypes regulated by the genes, determination of mutations present in the genome and in the production of proteins expressed from the respective genes for commercial use. Genetic engineering requires the use of recombinant DNA technology in order to carry out genetic manipulation of an organism. Genetic engineering can also be used to generate mutations of genes, whose functions are not known (Gaj, Gersbach, and Barbas). Various types of products like insulin, industrial enzymes and even the human growth hormone has been produced by the use of genetically modified organisms (GMOs). Genetically modified crops can also be produced using this technique (Bawa and Anilakumar). The overall purpose of this report is to generate the genomic DNA library of B. subtilis and carrying out the necessary steps to determine its efficacy. Results Lab 3 results The concentration of the B. subtilis genomic DNA isolated in Lab 2 was 44ng/l. EcoRI and HindIII restriction enzymes were used to digest the genomic DNA. The digested fragments were then ligated into the EcoRI and HindIII digested empty vector pUC18 to obtain recombinant plasmids carrying the various genetic regions of the B. subtilis genome. The recombinant plasmids thus obtained were introduced into the Escherichia coli DH5 cells. Restriction digestion of the genomic and plasmid DNA was carried out at 37C for 1 hour, then incubation at 80 C for 10 minutes was carried out to inactivate the restriction enzymes. The digestion products were at first heat inactivated at 45 C for 5 minutes to remove the reannealed digested products. This was followed by the actual ligation step at 18 C for 30 minutes. The ligation products were incubated at 65 C for 10 minutes to inactivate the ligase. The ligated products were transformed into E. coli DH5 cells plated on X-gal, IPTG and Ampicillin conta ining LB agar plates. Lab 4 results The ratio of the blue to white colonies were 3:8. The control plates used were the digested and re-ligated pUC18 plates, which gave rise to blue colonies due to the absence of an insert. The non-transformed and no T4 DNA ligase plates did not show the presence of colonies, while an already prepared recombinant plasmid was used as the positive control, which gave rise to white colonies (Apppendix, Figures 1-4). The recombinant clones obtained in the experimental plates were then used to isolate the recombinant plasmid. A white recombinant colony was selected. The concentration of the isolated plasmid DNA was 621.1ng/l and the 260:280 ratio was 2.12. The isolated recombinant plasmid was single digested with HindIII and double digested with EcoRI/HindIII. This helped to confirm the presence of the insert. Lab 5 results The distances travelled by the bands in the DNA ladder were calculated (Appendix, Table 1, Figure 5) and plotted with respect to the length of the DNA bands in base pairs. The X- axis of the standard curves generated represents the DNA length in base pairs and the Y- axis represents the distance travelled in mm. A logarithmic trendline was generated in case of both the standard curves (Appendix, Figures 6 and 7). Calculations using the equations in the standard curves were done to determine the sizes of the DNA bands in the gel (Appendix, Figure 5). The sizes of the DNA bands are provided in Table 2 (Appendix). In gel 1, the pUC 18 plasmid had three bands of sizes 12088, 5115 and 4023bp, respectively. The double digested pUC18 and the genomic DNA lanes showed one band of sizes approximately 2697bp and 13359bp, respectively. The control uncut recombinant plasmid had 4 bands of sizes 12088, 5115, 4023 and 2440.6bp. The single digested plasmid had one band of size 4769bp. The double digested control plasmid produces 2 bands of sizes 3165 and 1353bp, respectively (Appendix, Figure 5). In gel 3, the genomic DNA had one band of size 11849bp. The uncut plasmid had 2 bands of sizes 3605 and 2208bp. The single digested plasmid had one band of size 3605bp. The double digested product had 2 bands of sizes 2697 and 916bp. The foreign DNA insert was approximately 916bp (Appendix, Figure 5). While the single digested product was 3605bp approximately, the double digested product adds up to 3613bp. The single and double digestion of the control recombinant plasmid yielded one and two bands, respectively (A ppendix, Figure 5). Discussion This report describes the preparation of a genomic DNA library. Digestion and ligation of the genomic DNA inserts into the vector pUC18 yielded the recombinant clones. The number of white colonies were more than the number of blue colonies. The recombinant plasmid containing colonies were white because the lacZ gene present in the multiple cloning site (MCS) of the vector gets disrupted due to the addition of the insert (Davis). The blue colonies carried the empty vectors, which were obtained due to their undergoing only single digestion. As a result, they were able to break down the X-gal substrate giving rise to the blue color. This is because the lacZ gene remained intact producing functional beta galactosidase. Moreover, the double digestion of the recombinant plasmid yielded DNA bands of sizes 2697 and 916bp, which are the vector and insert bands, respectively. Additionally, the single digested product gave a single band of size 3605bp. The problems that were encountered were the absence of colonies. Absence of colonies can be due to improper plasmid and genomic DNA purification, which can hamper the digestion step (Surzycki). Star activity can result in non-specific digestion of the genomic and plasmid DNAs (Pingoud, Wilson and Wende). Moreover, restriction enzyme inactivation is crucial otherwise it can interfere with the ligation steps. Lastly, proper generation of competent cells are necessary for successful transformation (Tu et al.). Other interesting results that were obtained is the presence of white colonies in the pUC 18 plates and blue colonies in the experimental plate. The blue colonies in the experimental plate indicates the inappropriate digestion of any one of the enzymes, thereby resulting in re-ligation of the vector. The control plate containing pUC18 is expected to produce blue colonies, however, mutations in the lacZ gene can case the appearance of white colonies. Additional experiments includes polymerase chain and cycle sequencing to confirm the presence of the insert in the desired vector (Hoseini and Sauer). The genomic DNA library can be used identify genes that express commercially valuable protein products. Genomic DNA libraries can also be used to identify genetic alterations of an organism and also identify the genetic regulators that modulate an organisms genetic circuitry. The purpose is to overexpress the desired gene and produce large quantities of proteins, which will be subjected to further purification. The blue white screening is based on the theory of -complementation. lacZ encodes the enzyme -galactosidase, which is a tetramer having 2 and 2 fragments. E. coli cells that lack the fragment, produce non-functional -galactosidase. However, the fragment can be introduced by the introduction of a plasmid expressing the fragment in trans. The lacZ gene present in the MCS gets disrupted by the addition of an insert (Blau and Wehrman). IPTG and X-gal is added to the LB media. IPTG acts as the gratuitous inducer and X-gal functions as the chromogenic substrate. Non-functional LacZ cannot degrade the substrate and produce white coloration, while functional LacZ degrades the substrate to produce blue coloration. The recombinant plasmids are identified by the white coloration of the colonies (Chaudhuri). DH5 cells are M15 strains, where 11-41 amino acid residues ( fragment) from the N-terminal of LacZ is deleted and subsequently the residual fragment is inactive. Thus, the DH5 strain i s suitable for such a screening as blue colonies will appear only when a plasmid expressing the fragment is introduced (Aguilera and Aguilera-Gmez). Reference List Aguilera, Margarita, and Jess Manuel Aguilera-Gmez. "chapter seventeen."Applied Molecular Biotechnology: The Next Generation of Genetic Engineering(2016): 405. Bawa, A. S., and K. R. Anilakumar. "Genetically modified foods: safety, risks and public concernsa review."Journal of food science and technology50.6 (2013): 1035-1046. Blau, Helen M., and Thomas S. Wehrman. "Detection of protein translocation by beta-galactosidase reporter fragment complementation." U.S. Patent No. 8,586,294. 19 Nov. 2013. Capozzi, Vittorio, et al. "Lactic acid bacteria producing B-group vitamins: a great potential for functional cereals products."Applied microbiology and biotechnology96.6 (2012): 1383-1394. Chaudhuri, Keya.Recombinant DNA Technology. The Energy and Resources Institute (TERI), 2013. Davis, Leonard.Basic methods in molecular biology. Elsevier, 2012. Gaj, Thomas, Charles A. Gersbach, and Carlos F. Barbas. "ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering."Trends in biotechnology31.7 (2013): 397-405. Hoseini, Sayed Shahabuddin, and Martin G. Sauer. "Molecular cloning using polymerase chain reaction, an educational guide for cellular engineering."Journal of biological engineering9.1 (2015): 2. Logan, Niall A., and Paul De Vos. "Bacillus."Bergey's Manual of Systematics of Archaea and Bacteria(2015). Pingoud, Alfred, Geoffrey G. Wilson, and Wolfgang Wende. "Type II restriction endonucleasesa historical perspective and more."Nucleic acids research42.12 (2014): 7489-7527. Surzycki, Stefan.Basic techniques in molecular biology. Springer Science Business Media, 2012. Tu, Qiang, et al. "Room temperature electrocompetent bacterial cells improve DNA transformation and recombineering efficiency."Scientific reports6 (2016)

вторник, 3 декабря 2019 г.

My Mother Said I Never Should Essay Example

My Mother Said I Never Should Essay When looking for my contemporary monologue I at first wasnt sure what kind of monologue I wanted, I knew I wanted one which showed quite a bit of emotion but as to which monologue I should of chose I was stuck on. I eventually found one monologue its called my mother said I never should by Charlotte Keatley. The play is about difficult relationships between mothers and daughters. It goes forwards and backwards between 1905 and 1987. It shows the lives of four generations of women, their loves, expectations and choices throughout their lives. They are set against the huge social changes on the twentieth century. In the scene from which my monologue came from it is in 1987, Jackie is in her mid-thirties and she runs an art gallery in manchester. She has a very successful life but has a very painful secret. In the the early seventies at the age of nineteen she gave birth to an illegitimate daughter Rosie. She couldnt handle the demands of single parenthood after Rosies father moved away with another woman. She then had to give Rosie up under pressure from her mother, and so she was brought up by her grandparents. We will write a custom essay sample on My Mother Said I Never Should specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on My Mother Said I Never Should specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on My Mother Said I Never Should specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Later on one Margaret who Rosie thought was her mother had just died from cancer, Rosie was going through paperwork only to find her birth certificate. That is when she found out that Jackie was her actual mother and Jackie then trys to explain herself. Jackie- How dare you! (Goes to hit Rosie but cannot. ) Youre at the centre of everything I do! (Slight pause. ) Mummy treated me as though Id simply fallen over and cut my knee picked me up and said youll be all right now, it wont show much. She wanted to make it all better. (Quiet. ) †¦ She was the one who wanted it kept secret †¦ I wanted you, Rosie. Angry. ) For the first time in my life I took care of myself refused joints, did exercises, went to the clinic. (Pause. ) Its a girl. (Smiles irresistibly. ) After youd gone I tried to lose that memory. (Pause. Effort. ) Graham †¦ your father. (Silence. ) He couldnt be there the day you were born, he had to be in Liverpool. He was married. (Emphatic. ) He loved me, he loved you, you must believe that! (Pause. ) He said hed leave his wife, but I knew he wouldnt; there were two young children, the youngest was only four †¦ wed agreed, separate lives, I wanted to bring you up. He sent money. (Pause. ) I took you to Lyme Park one day, I saw them together, across the lake, he was buying them ice creams, his wife was taking a photo. I think they live in Leeds now, I saw his name in the Guardian last year, an article about his photographs †¦ (Pause. ) It was a very cold winter after you were born. There were power cuts. I couldnt keep the room warm; there were no lights in the tower blocks; I knew he had an open fire, it was trendy; so we took a bus to Didsbury, big gardens, pine kitchens, made a change from concrete. I rang the bell. (Stops. ) A Punjabi man answered, said he was sorry †¦ theyd moved. By the time we got back to Moss Side it was dark, the lift wasnt working (Stops. ) That was the night I phoned Mummy. (Difficult. ) Asked her. (Pause. ) I tried! I couldnt do it, Rosie. (Pause. ) It doesnt matter how much you succeed afterwards, if youve failed once. (Pause. ) After youd gone †¦ I kept waking in the night to feed you †¦ A week †¦ in the flat †¦ Then I went back to art school. Sandra and Hugh thought I was inhuman. I remember the books hat came out that winter how to succeed as a single working mother fairytales! (Pause. ) Sandra and Hugh have a family now. Quite a few of my friends do. (Pause. ) I could give you everything now. Rosie? †¦ In this monologue I could see that the short sentances punctuated by pauses and silences, bursts of anger and overemphasis show how difficult it is for Jackie. I thought about how hard it must be to tell your daughter that you gave her away, Jackie has now lost her mother and wasnt there for her in time to say goodbye. Now her relationship with her daughter is at stake. Jackie who is guilt and grief stricken she trys to offer a fractured explanation for what she did. You can see that she is still trying to hold on to the myth that the father of Rosie still loves them both as she trys to get Rosie to also belive it. I thought that this monologue was a very good emotional one. I feel that I could perform this fairly well as I can look at the emotion and I can portray it fairly well. The second monologue I looked at was called Cant stand up for falling down by Richard Cameron. The play is set in a coal mining town in South Yourkshire on a stretch down by the River Don. It is about three young women, all deeply affected by the death of a local man, all with a good reason to hate the heartless town bully, Royce Boland. The story starts to unfold through looking at each of their perspectives and series of monologues which are often addressed directly to the audience. Lynette is a working class Yorkshire woman of twenty-two. She married Royce at eighteen but has lived to regret it. She was brought up to belive that marriage is sacred and you must try your hardest to make it work. But Royce is a violent drunk man who beats, demeans and terrorises her. Royce forced her to give up her job in the coal board offices and now she stays home cooks, cleans and sometimes works in their fish tackle shop. Every now and then she shows a bleak sense of humor. There are moments throughout where she is sorry and things improve for a while. It shows in her monologue that it is true hatred that runs through her for him. My Mother Said I Never Should Essay Example My Mother Said I Never Should Paper My Mother Said I Never Should follows the story of four generations of women, a great-grand-daughter a daughter, a mother and a grandmother, grappling with social forces that threaten to split them apart as the twentieth century grows older. Widely studied on Drama syllabuses the play moves back and forth through the lives of the women, it sets the enormous social changes of this century against the needs of the individual. After reading the play mentioned, my initial sentiments on it were that I highly enjoyed it because I found it highly engaging and very original and abstract by its use of different time frames and using the waste-ground scenes as a type of limbo where time is of no consequence and causes the reader to primarily focus on the relationships between each woman. Nonetheless, I found myself becoming highly confused whilst reading the play due to the constant switching between time frames. In my opinion, I think the playwright is trying to convey the hidden relationships between women within the same family. She tries to show how each of the women interact with each other and how the role of women in society can change within each generation. Keatley tries to focus primarily on women and I feel that the men within the play were portrayed as the draw-backs of the women, for example, in the play Margaret becomes highly depressed due to her failing marriage with Ken. We will write a custom essay sample on My Mother Said I Never Should specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on My Mother Said I Never Should specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on My Mother Said I Never Should specifically for you FOR ONLY $16.38 $13.9/page Hire Writer Another example would be that Jackies career and opportunity for the future couldve been destroyed because she fell pregnant with Neils baby, and soon after, Neil abandons her. The message the playwright was trying to put across is the how the unique bonds between mothers and daughters survives behind closed doors, and how secrecy may slowly destroy a family. The practical problems I foresaw in performing this play were being able to mentally as well as physically change myself into a female child, teenager, adult or old woman. I also found it challenging to be able to relate to each character and infuse a little part of my personality to each of them. I found I had these problems because I have not experienced some of the things the characters within the play had done. I managed to overcome this by remembering certain plays/television programmes/films I have seen that cover the certain subjects that each character was going through, for example when Jackie fell pregnant, I thought of Lolita by Stanley Kubrick when Lolita falls pregnant at 16/17.